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This workshop will introduce the concepts behind transcriptomics with NGS data and how to analyze this data in Galaxy. Specifically, this workshop will focus on
de novo transcriptome reconstruction of RNA-seq data with the following goals:
- comprehensive identification of all transcripts across an experiment
- appropriately annotating classes of transcripts
- generating abundance estimates across a transcriptome
- significance testing of differentially expressed transcriptshttps://vimeo.com/494282844
- visualisation of reads and transcript structures
Prerequisites